Klf2 shrna

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August undefined, 2024

WebShRNA of mouse Klf2 were inserted into pLKD-CMV-G and PR-U6-shRNA vector. The sequences of shRNA are listed in Table S3. Lentiviral package Human embryonic kidney (HEK) 293T cells (ATCC, CRL-1573) were transfected with the vector plasmid, the lentiviral packaging constructs pCMV.DR8 and pMD2.G (Plasmid #12259, Addgene). WebSep 8, 2015 · Klf2 and Tfcp2l1 Knock Down Impairs 2i-Mediated mESC Self-Renewal (A) qRT-PCR analysis of Klf2 expression in Klf2 shRNA knockdown 46C mESCs. Data represent mean ± SD of three biological replicates. ∗∗ p < 0.01 versus scramble shRNA. (B) Western blot analysis of KLF2 expression in Klf2 shRNA knockdown 46C mESCs.

Transcriptional repression of Kruppel like factor-2 by the …

WebMar 2, 2015 · The patients were divided into a low KLF2 expression group (n=59) and a high KLF2 expression group (n=21) according to whether their samples showed up or downregulation of TINCR compared with the ... WebFeb 10, 2008 · Normal undifferentiated ES colonies with positive alkaline phosphatase staining were maintained after knockdown using two shRNA constructs targeting different regions of Klf2 or Klf5, respectively. state farm in oshkosh wi

Klf2 Sigma-Aldrich

WebOct 31, 2024 · For KLF2 knockdown, a human KLF2 gene-specific silencing and control lentivirus vector short hairpin RNA (shRNA) was developed, and the corresponding lentivirus particles were produced. Then, the KLF2 and control knockdown lentiviruses were concentrated using a density gradient centrifuge and titrated to determine virus efficiency. Webactivity of KLF2 and ATF2 transcription factors, resulting in quiescent and healthy endothelium.3–7 However, as other molecular factors may also play a role, we recently established the speciﬁc gene expression in endothelium overlaying human atherosclerotic lesions, to identify genes with a potential to modulate intimal accumulation of ... WebMay 3, 2010 · Klf2-expressing retrovirus and shRNA-expressing lentivirus. A mouse Klf2-expressing retroviral vector was prepared by PCR-based cloning of mouse Klf2 cDNA into a mouse stem cell virus–based vector and IRES2-EGFP that are expressed under the viral endogenous promoter. The virus was assembled in Phoenix cells provided by Garry Nolan … state farm in owensboro ky

Silencing of Kruppel-like factor 2 by the histone ... - Nature

Klf2 and Tfcp2l1, Two Wnt/β-Catenin Targets, Act Synergistically …

WebSep 1, 2024 · For shRNA-mediated knockdown of Klf2 and Snd1, DNA fragments targeting Klf2 and Snd1 were subcloned into pLKO.1-TRC vector (Addgene). Lentiviral vectors were co-transfected into HEK293T cells together with pCMV-VSV-G (Addgene) and psPAX2 (Addgene) plasmids using Lipofectamine 3000 reagent (Invitrogen), and viruses were … WebWEE1-shRNA vectors and the NC vectors were transfected into MDA-MB-231 cells, and WEE1 expression was detected by Western blot to screen the most effective of the two shRNAs. ... First, abnormal downregulation of KLF2 expression in tumors mainly results from epigenetically silence mediated by histone methylation. 45, 46 Since no small molecule ... state farm in pahrumpWebMay 7, 2024 · These results confirm that this dual transduction protocol does not affect A20's ability to increase KLF2, KLF4, and eNOS expression levels. Transduction of HCAEC … state farm in paw paw mi

"WebJan 1, 2024 · Endothelial KLF2 mediates the inhibitory effects of simvastatin on TGFβ stimulated cardiac microvasculature endothelial cells condition medium (mCMVEC-CM) enhanced migration, proliferation and... " - Klf2 shrna

Klf2 shrna

Endothelial UCP2 Is a Mechanosensitive Suppressor of …

WebJul 28, 2024 · Overexpression of KLF2 by adenovirus KLF2 (Ad-KLF2) in HUVECs reversed the inhibitory effect of OSS on UCP2 mRNA expression , suggesting that KLF2 can increase UCP2 expression. However, … WebMay 8, 2008 · KLF2 induces the inhibitory SMAD7 and reduces nuclear levels of the AP-1 member c-Jun, thereby fully suppressing TGF-β–driven gene expression. 6 A third cluster showed enrichment for the antioxidant response element (ARE), suggesting a role for nuclear factor erythroid 2-like 2 (Nrf2) that binds the ARE. 3

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WebFAM83A and DDIT4 expression was knocked-downed in leukemia cells via lentivirus-shRNA. Western blotting, RT-qPCR, cell cycle analysis and apoptosis assays were used to determine the effect of lovastatin-induced growth suppression in leukemic cells in vitro. ... We further show that KLF2 induction by lovastatin is responsible for lower ... WebNov 22, 2024 · Endothelial–mesenchymal transition occurs during intimal hyperplasia and neointima formation via mechanisms that are incompletely understood. Endothelial MAPK7 signaling is a key mechanosensitive fac...

WebApr 11, 2024 · The shRNA for human CUX1 based on the previous study [28] was packaged into lentivirus by GenePharma Co., Ltd. (Shanghai, China). Cells were transfected with lentivirus carrying shRNA-CUX1 or negative control sequences for 24 h and then cultured in a new DMEM medium for 48 h. ... Klf2, Klf4, and Klf6 were found to be the key TFs in … WebMEF2A protein and mRNA are more abundant in p66shc-knockout MEFs, resulting in greater occupancy of the KLF2 promoter by MEF2A. In endothelial cells, the increase in KLF2 and thrombomodulin protein by shRNA-induced decrease in p66shc expression is partly abrogated by knockdown of MEF2A.

WebMay 7, 2024 · In select experiments, HCAEC were first transduced for 24 h with a rAd expressing a human KLF2 silencing short hairpin (Ad-h-KLF2-shRNA) or a control rAd comprising a scramble short shRNA sequence (Ad-GFP-U6-shRNA) at a MOI of 100 (Vector Biolabs, Malvern, PA). WebApr 4, 2024 · RNAseq analysis of lovastatin treated leukemic cells revealed a strong induction of the tumor suppressor gene KLF2. Accordingly, lentivirus-mediated …

WebSilencing of KLF2 with KLF2 shRNA abolished the effects of laquinimod in inhibiting the expression of endothelial adhesion molecules and attachment of U937 monocytes to …

WebWestern blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 expression was … state farm in odessa texasWebApr 15, 2024 · However, SK-MES-1 cells transfected with KLF2 shRNA#3 or shRNA#4 had a significant decrease in the G0/G1 phase but the cell cycle progressed into the S and G2/M phase (Figure 5I). To further explore the underlying mechanisms of KLF2 re-expression involved in cell cycle arrest at the G0/G1 phase we performed western bolt analysis of p15 … state farm in pinehurst ncWebDiabetic mice injected with KLF2 shRNA-lentivirus or control virus were treated with 20 mg/kg phloretin. Glucose, lipid profile, aortic atheroma, and endothelial nitric oxide synthase (eNOS) expression were detected. Phloretin retained endothelial function by KLF2-eNOS activation under hyperglycemia. state farm in purvis msWebApr 15, 2024 · Western blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 … state farm in rawlins wyWebApr 15, 2024 · Western blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 … state farm in rehoboth deWebFor KLF2 knockdown, a human KLF2 gene-specific silencing and control lentivirus vector short hairpin RNA (shRNA) was developed, and the corresponding lentivirus particles were produced. Then, the KLF2 and control knockdown lentiviruses were concentrated using a density gradient centrifuge and titrated to determine virus efficiency. state farm in rio rancho nmWebWe further show using KLF2 shRNA that the inhibitory eect of NMP on endothelial inammation and subsequent monocyte adhesion is KLF dependent. Enhancing KLF expression and activity improves state farm in platteville wi