Intratracheal mouse
WebMay 1, 2000 · Although the intratracheal instillation technique has been used quite extensively for administering fibers to the lungs of rats, hamsters, and mice, only one … WebDec 2, 2024 · Infectious pneumonia induced by multidrug resistant (MDR) Acinetobacter baumannii strains is among the most common and deadly forms of healthcare acquired …
Intratracheal mouse
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WebJun 24, 2024 · Streptococcus pneumoniae causes many people to suffer from pneumonia, septicemia, and other diseases worldwide. To identify the difference in susceptibility of and treatment efficacy against S. pneumoniae in three ICR mouse stocks (Korl:ICR, A:ICR, and B:ICR) with different origins, mice were infected with 2 × 106, 2 × 107, and 2 × 108 CFU … WebApr 1, 2024 · The mouse model of ALI was established by intratracheal administration of LPS from E. coli at a dose of 10 mg/kg body weight, while intratracheal LPS administration at a dose of 20 mg/kg body weight was employed in survival studies, since intratracheal injection with a low dose of LPS could induce lung injury only and was not adequate to …
WebMar 1, 2024 · The mice in the oral group received 100 mg/kg Green Fluorescent PS-MPs daily by gavage, while those in the intratracheal administration group were given 12 mg/kg Green Fluorescent PS-MPs every day. The mice were administered for 7 consecutive days and then sacrificed to obtain the lungs, livers and kidneys. WebAfter intratracheal treatment, the mice were kept in an upright position for 10 min to allow suf-ficient spreading of the fluid throughout the lungs. Thirty mice were divided into four experimental groups. LPS 1 group (n 10): intratracheal exposure to 5 g LPS; mice were killed 1 wk af-ter the final intratracheal instillation (1-wk recovery ...
WebWe describe a method for simple and rapid intratracheal intubation in mice for mechanical ventilation, using a self-built plastic support to facilitate the intubation procedure. General … WebIntratracheal instillation is the introduction of a substance directly into the trachea.It is widely used to test the respiratory toxicity of a substance as an alternative to inhalation in animal testing. Intratracheal instillation was reported as early as 1923 in studies of the carcinogenicity of coal tar.Modern methodology was developed by several research …
WebJul 15, 2024 · The mice were housed in micro-isolator cages and were provided diet and water ad libitum. ... Japan) with a 20× objective lens. Intratracheal instillation was performed to collect BAL fluid with 400 μL of pre-cooled PBS. The lavage fluid was centrifuged, and the supernatants were used for cytokine measurements.
WebThe mice were immunized by aerosolized intratracheal inoculation or subcutaneous injection (hereafter, i.t. and s.c., respectively) on days 0, 21, and 42 of the trial. The liquid and dry powder formulations were administered with MicroSprayer Aerosolizer or Dry Powder Insufflator (Huironghe Company, Beijing, China), respectively, following … lupita colonWebDec 20, 2016 · Here we describe an intratracheal injection method that was enabled by a three dimensional printing of a custom platform. This updated technique improved the overall ergonomics of intratracheal injection in mice, minimizing the risk of human injury and implementing the 3R (replacement, reduction and refinement) principle in mouse … lupita cabrera photographyWebOct 1, 2016 · Notably, the mouse model of respiratory infection, induced by intratracheal injection and used in our work, differs from the models generated previously that reproduced the systemic infection with S. aureus introduced by an intraperitoneal injection. 30 In our model of acute pneumonia, it should be pointed out that mice with DHR activity as low ... lupita corbeillupita coronelWebOct 1, 2010 · Bleomycin is used extensively to model lung fibrosis in mice and can be given by multiple routes: intratracheal, intranasal, subcutaneous, intraperitoneal, or intravenous. The subcutaneous, intraperitoneal, or intravenous routes deliver the drug systemically and provide clinically relevant models for human forms of bleomycin lung … lupita corralWebIn the mouse model, the increased number of inflammatory cells in the bronchoalveolar lavage fluid and the degree of lung fibrosis observed in BLM-treated mice were significantly reduced by MSC treatment, which tended to be more prominent with intravenous administration than intratracheal administration. lupita commercial streetWebSpecific information for mice and rats can be found in Tables 1 and 2 at the bottom of this page. C. ... Upper Respiratory Tract – Intratracheal: Maximum volume to be injected in adult mice is 50 µl. This method, requiring anesthesia, must be described in detail in the IACUC protocol. lupita comida