Chimeric chalcone synthase

WebThe present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the m WebJSTOR Home

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WebApr 26, 2024 · Chalcone synthase (CHS) catalyzes the first step of flavonoid biosynthesis by directing carbon flux from general phenylpropanoid metabolism to flavonoid pathway. Despite extensive characterization of its function and transcriptional regulation, the molecular basis governing its posttranslational modification is enigmatic. WebA chimeric gene was constructed containing the light‐inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence … how to reset breaker switch https://anthonyneff.com

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WebMay 3, 2011 · Chalcone synthase (CHS, EC 2.3.1.74) is a key enzyme of the flavonoid/isoflavonoid biosynthesis pathway. Besides being part of the plant developmental program the CHS gene expression is induced in … WebIn Basic medium (base catalysis), the chalcone is produced from the aldol product via dehydration in (an enolate) - mechanism, while in acidic medium (acid catalysis, it is produced via (an... WebMar 20, 2001 · chalcone synthaseA ( chsA) into Petunia induces PTGS as indicated by white flower tissue 11, 12, 13. To determine whether dsRNA triggers chsA PTGS, the constructs 35S-IR.chs and IR.chs were transformed into Petunia. They carry two chsA cDNAs that are arranged as inverted repeats (IR). north carolina public schools jobs

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Category:Chalcone synthase and its functions in plant resistance

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Chimeric chalcone synthase

REVIEW ON CHEMICAL-BIOLOGICAL FIELDS OF CHALCONE COMPOUNDS …

WebJun 1, 1992 · We developed an Agrobacterium-mediated leaf disk transformation method for chrysanthemum. We introduced a chimeric chalcone synthase (CHS) gene isolated from chrysanthemum into cv. Moneymaker (pink type) to produce white-flowered plants. WebOct 21, 2013 · The I locus is a 27-kb inverted repeat cluster of chalcone synthase genes CHS1-3-4 that mediates siRNA down-regulation of CHS7 and CHS8 target mRNAs during seed development leading to yellow seed coats lacking anthocyanin pigments. Here, we report small RNA sequencing of ten stages of seed development from a few days post …

Chimeric chalcone synthase

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WebApr 1, 1990 · Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. C. Napoli, C. Lemieux, R. … WebMay 3, 2011 · Chalcone synthase (CHS, EC 2.3.1.74) is a key enzyme of the flavonoid/isoflavonoid biosynthesis pathway. Besides being part of the plant developmental program the CHS gene expression is induced in plants under stress conditions such as UV light, bacterial or fungal infection. CHS expression causes accumulation of flavonoid and …

WebThe primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin. Catalytic activity 4-coumaroyl-CoA + 2 H+ + 3 malonyl-CoA = 2',4,4',6'-tetrahydroxychalcone + 3 CO2 + 4 CoA PROSITE-ProRule annotation WebSep 1, 2016 · DPPH (1,1–diphenyl-2-picrylhydrazyl) radical scavenging activity was measured for all the chalcones (2a–2e).Among them, compounds 2a, 2b, 2c, 2d and 2e …

WebMar 21, 1997 · Transgenic Petunia plants with a chsA coding sequence under the control of a 35S promoter sometimes lose endogene and transgene chalcone synthase activity and purple flower pigment through posttranscriptional chsA RNA degradation. In these plants, shorter poly(A)+ and poly(A)− chsA RNAs are found, and a 3′ end–specific RNA … WebApr 1, 1990 · Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. Napoli C1, Lemieux C, Jorgensen R Author information Affiliations 1 author 1. DNA Plant Technology Corporation, 6701 San Pablo Avenue, Oakland, California 94608. ORCIDs linked to this article

WebDec 13, 2024 · The chalcone synthase superfamily of type III polyketide synthases. ... A study utilizing both mutant and BBS/STS chimeric enzymes attempted to elucidate the structural basis for this unusual substrate …

WebChalcone synthase or naringenin-chalcone synthase is an enzyme ubiquitous to higher plants and belongs to a family of polyketide synthase enzymes known as type III PKS. … how to reset brake warning lightWebChalcone synthase is a dimeric protein with Mr 78 000–88 000 and probably two identical subunits. The pH optimum of the reaction is in the range 7.5–8.5 for 4-coumaroyl-CoA. … north carolina puppet theatreWebIn the chalcone synthase gene of Antirrhinum majus (snapdragon), 150 base pairs of the 5' flanking region contain cis-acting signals for UV light-induced expression. A nuclear factor, designated CG-1, specifically recognizes a hexameric motif with internal dyad symmetry, CACGTG, located within this light-responsive sequence. how to reset breakerWebJSTOR Home north carolina pulled pork recipes crockpotWebNational Center for Biotechnology Information how to reset breckwell pellet stoveWebA chimeric gene was constructed containing the light‐inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence from Tn5 as a reporter gene (NPTII) and the termination region from chs gene 1 from Petroselinum hortense. how to reset brinks alarm panelWebJan 11, 2024 · Furthermore, SMAT sequencing created 429,955 reads of inserts, where 406,722 of them were full-length non-chimeric reads, and 131,357 non-redundant isoforms were produced. Abundant differentially expressed genes were found in leaves under different light qualities by RNA-sequencing. ... Chalcone synthase (CHS) is a catalytic … north carolina purchase tax